例句與造句

1. According to csfv ' s e2 gene sequence published in genbank and the sequence of p . pastoris expression vector ppic9 ' s multiple cloning site ( mcs ) , a pair of primers were designed with oligo and primers . o softwares
   將該基因片段先克隆到pmd18 - t載體上，并進(jìn)行了酶切、 pcr鑒定和測(cè)序分析，陽性重組質(zhì)粒命名為t - e2 。
2. Construction of two vectors containing different plasmid original replicon this work constructed four resolution shuttle vectors , pbmb1205 , pbmb1205r , pbmb1206 and pbmb1206r . there are multiple clone sites between two copies of res sites
   解離載體的構(gòu)建和性能利用來源于不同質(zhì)粒上的質(zhì)粒復(fù)制起始區(qū)ori44和ori1030構(gòu)建了4個(gè)解離載體。
3. The recombinant transfer vector pbacpak - hbmp was constructed by insertion of the hbmp coding sequences into the multiple cloning site of transfer vector pbacpak . 8 . bmn cell line was co - transfected with pbacpak - hbmp plasmid and linearized baculovirus bacpak6 dna by dosper agent
   將克隆到的hbmp基因通過適當(dāng)?shù)拿盖胁迦氲睫D(zhuǎn)移載體質(zhì)粒pbac - pak8的多克隆位點(diǎn)中，獲得重組轉(zhuǎn)移載體質(zhì)粒pbacpak - hbmp 。
4. The interest gene was inserted in the - tha l . tho 1 multiple cloning sites of donor plasimd pfastbachtb of baculovirus expression system . after analysis by restriction endonuclease and pcr , the recombinant donor plasmid gpl - fast was transforn1ed to the competent celi dhi0bac which contalns the bacmid and the helper plasmid , the recombinant bacmid gpl - bac was acquired which would express the vpl of gpv strain h l
   同時(shí)將該目的基因插入到桿狀病毒表達(dá)系統(tǒng)的供體質(zhì)粒pf _ ( ast ) b _ ( ac ) htb的xba 、 xho多克隆位點(diǎn)間，經(jīng)酶切、 pcr鑒定后，將重組的供體質(zhì)粒gp1 - fast轉(zhuǎn)化到含有桿狀病毒和輔助質(zhì)粒的dh10b _ ( ac )感受態(tài)細(xì)胞中，獲得了表達(dá)gpvh1株vp1的重組桿狀病毒gp1 - bac 。
5. A bt - e . coli shuttle vector pht315 was deleted its replication region of bt , then constructed a novel vector named pht315 - 1 which composed a multiple cloning site , erythromycin and ampicillin - resistance marker and could only replicated in e . coli . used pht315 - 1 , a 5273 bps dna fragment carrying a novel bt plasmid replicon was isolated and registered in genbank as ay278324 . sequence analysis showed that there were at least three orf ( open reading frame ) in the cloned dna encoding 501 , 333 , 183aas . orfl had 98 % identities with replicating related protein ori43 of bt strain hd263 . the others were no homology to any published bt replicating related protein . after continuous cultured for 70h at 30 c without antibiotic selecting press . the stability of plasmid carrying cloned replicon in bt acrystalliferous mutant strain hd73 cry was more than 98 % . and growth curve also showed that the novel replicon was stable and could replicate normally
   進(jìn)一步序列分析表明該復(fù)制區(qū)至少有3個(gè)較大的orf ，分別編碼501 ， 333 ， 183個(gè)氨基酸。其中orf1蛋白序列與hd263復(fù)制蛋白o(hù)ri43的同源性為98 ，而另外兩個(gè)orf和genbank己公布的bt復(fù)制相關(guān)蛋白無同源性。 30連續(xù)培養(yǎng)72h ，復(fù)制區(qū)質(zhì)粒在bt無晶體突變株hd73cry ~ -中穩(wěn)定性達(dá)98以上， 30h生長曲線也表明該復(fù)制區(qū)能夠在bt中穩(wěn)定復(fù)制和遺傳，對(duì)受體菌株無明顯不良影響。
6. It's difficult to find multiple cloning sites in a sentence. 用multiple cloning sites造句挺難的
7. Sequence analysis shows that they share 98 . 75 % similarity at the dna , and 98 . 67 % at the protein level . ns2 gene was cloned into the multiple cloning sites of prokaryotic expression vector pgex - 6p - l . the recombinant plasmid pgex - 6p - ns2 was constructed and transformed to the competent cell bl21 ( de3 ) plyss , positive bacterium strain was induced by iptg
   將ns2基因插入到原核表達(dá)性質(zhì)粒pgex - 6p - 1的ecor 、 bamh多克隆位點(diǎn)之間，將重組原核表達(dá)質(zhì)粒pgex - 6p - ns2轉(zhuǎn)化到bl21 ( de3 ) plyss感受態(tài)細(xì)胞中，獲得了表達(dá)ns2基因的陽性亞克隆重組子，在含amp的lb液體培養(yǎng)基中培養(yǎng)，經(jīng)iptg誘導(dǎo)表達(dá)，用sds - page分析表達(dá)產(chǎn)物。
8. A pair of primers were designed to amplify vpl gene by pcr according to the published sequence of gpv b strain in genbank . the product of pcr was 2 . 2 kb . after identification of pcr product by restriction endonuclease the interest gene was inserted in the tha i . tho i multiple cloning sites of prokaryotlc expression vector pproexhtb to construct the recombinant prokaryotic expression vector gpl - ppro of vp 1 of gpv strain h 1
   本試驗(yàn)參照genbank中發(fā)表的gpvb株基因序列設(shè)計(jì)并合成了擴(kuò)增gpvh1株vp1的一對(duì)引物，利用pcr技術(shù)擴(kuò)增出長約2 . 2kb的目的片段，經(jīng)酶切鑒定后，將其插入原核表達(dá)載體pproexhtb的xba 、 xho多克隆位點(diǎn)間，構(gòu)建了gpvh1株vp1的原核表達(dá)載體gp1 - ppro 。
9. As well as in eukaryocyte ( hepg2 and cos - 7 ) , then detect their antigenity as a basis study and explore of the choice of immunogen for preventive and therapeutic vaccines of hepatitis b . methods : the gene fragments coding 152aa ( si ) and 124aa ( s2 ) of the carboxyl terminus of hbsag were amplified by pcr from plasmid pecob6 with a pair of primers containing different endonuclease sites and were cloned into multiple cloning sites of plasmid pbks ( + )
   為乙型肝炎的預(yù)防和治療性疫苗免疫原的選擇進(jìn)行初步的研究和探討。方法：本研究利用聚合酶鏈反應(yīng)( pcr ) ，通過設(shè)計(jì)帶有不同酶切位點(diǎn)的一對(duì)引物，從質(zhì)粒pecob6特異性擴(kuò)增hbsag蛋白羧基末端152個(gè)氨基酸( s1 )和124個(gè)氨基酸( s2 )的基因片段，分別將二者克隆到質(zhì)粒pbks ( + )的多克隆位點(diǎn)，篩選重組克隆。